Characterization of the Surface Proteins of Influenza A (H5N1) Viruses Isolated from Humans in 1997–1998
Identifieur interne : 001A33 ( Main/Exploration ); précédent : 001A32; suivant : 001A34Characterization of the Surface Proteins of Influenza A (H5N1) Viruses Isolated from Humans in 1997–1998
Auteurs : Catherine Bender ; Henrietta Hall ; Jing Huang ; Alexander Klimov ; Nancy Cox ; Alan Hay [Royaume-Uni] ; Victoria Gregory [Royaume-Uni] ; Keith Cameron [Royaume-Uni] ; Wilina Lim [République populaire de Chine] ; Kanta SubbaraoSource :
- Virology [ 0042-6822 ] ; 1999.
English descriptors
- KwdEn :
- Teeft :
- Academic press, Acid change, Acid deletion, Amino, Amino acid, Amino acid differences, Amino acid residues, Amino acid sequence, Amino acid sequences, Amino acids, Antigenic, Antigenic characteristics, Avian, Avian influenza, Avian influenza viruses, Canine kidney, Chick embryo fibroblasts, Claas, Cleavage, Cleavage site, Deletion, Disease control, Embryonated eggs, Evolutionary relationships, Gene, Genetic reassortment, Glycosylation, Glycosylation site, Glycosylation sites, Growth characteristics, Hemagglutinin, Hemagglutinin gene, Hong kong, Horizontal distances, Host range, Human influenza, Human population, Infectious virus, Influenza, Influenza virus, Influenza virus neuraminidase, Influenza viruses, Kawaoka, Mdck, Mdck cells, Migratory waterfowl, Molecular mechanism, Neuraminidase, Neuraminidase gene, Nucleotide, Nucleotide differences, Nucleotide sequences, Outbreak, Pandemic, Pandemic influenza, Pathogenic, Pathogenic phenotype, Potential glycosylation site, Potential glycosylation sites, Same outbreak, Suarez, Subbarao, Subtypes, Surface proteins, Unique changes, Virol, Virus, Virus titer.
Abstract
Abstract: Influenza A (H5N1) viruses infected humans in Hong Kong between May and December, 1997. Sixteen viruses, including 6 from fatal cases, were isolated during this outbreak. Molecular analysis of the surface proteins genes encoding the hemagglutinin (HA) and neuraminidase (NA) of these H5N1 isolates, of a subtype not previously known to infect humans, are presented. The 16 human H5 HA sequences contain multiple basic amino acids adjacent to the cleavage site, a motif associated with highly pathogenic avian influenza A viruses. The phylogenetic relationship among both avian and human H5 hemagglutinins indicates that the human isolates are related directly to isolates that circulated among chickens in the live poultry markets in Hong Kong prior to and during the outbreak in humans. HA sequences from the human isolates and a recent chicken isolate represent a separate clade, within which there are two subgroups that are distinguishable antigenically and by the presence of a potential glycosylation site. Likewise the N1 neuraminidases of the human H5 isolates represent a clade that is evolutionarily distinct from previously characterized N1 neuraminidases. The recent human H5N1 virus NA genes are avian-like, indicating direct introduction from an avian source rather than evolution of a human N1 NA. All of the 16 human NA genes encode a shortened stalk due to a 19-amino acid deletion, also found in the recent avian H5N1 isolates from Hong Kong. Two unique amino acids were identified in the N1 NAs of the recent human isolates; however, it is not known if these residues influence host range. Neither the HA nor the NA genes of the human H5N1 virus isolates show evidence of adaptive changes during the outbreak. Although analyses of the surface protein genes of the H5N1 viruses from this outbreak did not provide immediate answers regarding the molecular basis for virulence, the analyses provided clues to potentially important areas of the genes worth further investigation.
Url:
DOI: 10.1006/viro.1998.9529
Affiliations:
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eggs</term><term>Evolutionary relationships</term><term>Gene</term><term>Genetic reassortment</term><term>Glycosylation</term><term>Glycosylation site</term><term>Glycosylation sites</term><term>Growth characteristics</term><term>Hemagglutinin</term><term>Hemagglutinin gene</term><term>Hong kong</term><term>Horizontal distances</term><term>Host range</term><term>Human influenza</term><term>Human population</term><term>Infectious virus</term><term>Influenza</term><term>Influenza virus</term><term>Influenza virus neuraminidase</term><term>Influenza viruses</term><term>Kawaoka</term><term>Mdck</term><term>Mdck cells</term><term>Migratory waterfowl</term><term>Molecular mechanism</term><term>Neuraminidase</term><term>Neuraminidase gene</term><term>Nucleotide</term><term>Nucleotide differences</term><term>Nucleotide sequences</term><term>Outbreak</term><term>Pandemic</term><term>Pandemic influenza</term><term>Pathogenic</term><term>Pathogenic phenotype</term><term>Potential glycosylation site</term><term>Potential glycosylation sites</term><term>Same outbreak</term><term>Suarez</term><term>Subbarao</term><term>Subtypes</term><term>Surface proteins</term><term>Unique changes</term><term>Virol</term><term>Virus</term><term>Virus titer</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Influenza A (H5N1) viruses infected humans in Hong Kong between May and December, 1997. Sixteen viruses, including 6 from fatal cases, were isolated during this outbreak. Molecular analysis of the surface proteins genes encoding the hemagglutinin (HA) and neuraminidase (NA) of these H5N1 isolates, of a subtype not previously known to infect humans, are presented. The 16 human H5 HA sequences contain multiple basic amino acids adjacent to the cleavage site, a motif associated with highly pathogenic avian influenza A viruses. The phylogenetic relationship among both avian and human H5 hemagglutinins indicates that the human isolates are related directly to isolates that circulated among chickens in the live poultry markets in Hong Kong prior to and during the outbreak in humans. HA sequences from the human isolates and a recent chicken isolate represent a separate clade, within which there are two subgroups that are distinguishable antigenically and by the presence of a potential glycosylation site. Likewise the N1 neuraminidases of the human H5 isolates represent a clade that is evolutionarily distinct from previously characterized N1 neuraminidases. The recent human H5N1 virus NA genes are avian-like, indicating direct introduction from an avian source rather than evolution of a human N1 NA. All of the 16 human NA genes encode a shortened stalk due to a 19-amino acid deletion, also found in the recent avian H5N1 isolates from Hong Kong. Two unique amino acids were identified in the N1 NAs of the recent human isolates; however, it is not known if these residues influence host range. Neither the HA nor the NA genes of the human H5N1 virus isolates show evidence of adaptive changes during the outbreak. Although analyses of the surface protein genes of the H5N1 viruses from this outbreak did not provide immediate answers regarding the molecular basis for virulence, the analyses provided clues to potentially important areas of the genes worth further investigation.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Bender, Catherine" sort="Bender, Catherine" uniqKey="Bender C" first="Catherine" last="Bender">Catherine Bender</name><name sortKey="Cox, Nancy" sort="Cox, Nancy" uniqKey="Cox N" first="Nancy" last="Cox">Nancy Cox</name><name sortKey="Hall, Henrietta" sort="Hall, Henrietta" uniqKey="Hall H" first="Henrietta" last="Hall">Henrietta Hall</name><name sortKey="Huang, Jing" sort="Huang, Jing" uniqKey="Huang J" first="Jing" last="Huang">Jing Huang</name><name sortKey="Klimov, Alexander" sort="Klimov, Alexander" uniqKey="Klimov A" first="Alexander" last="Klimov">Alexander Klimov</name><name sortKey="Subbarao, Kanta" sort="Subbarao, Kanta" uniqKey="Subbarao K" first="Kanta" last="Subbarao">Kanta Subbarao</name></noCountry><country name="Royaume-Uni"><noRegion><name sortKey="Hay, Alan" sort="Hay, Alan" uniqKey="Hay A" first="Alan" last="Hay">Alan Hay</name></noRegion><name sortKey="Cameron, Keith" sort="Cameron, Keith" uniqKey="Cameron K" first="Keith" last="Cameron">Keith Cameron</name><name sortKey="Gregory, Victoria" sort="Gregory, Victoria" uniqKey="Gregory V" first="Victoria" last="Gregory">Victoria Gregory</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Lim, Wilina" sort="Lim, Wilina" uniqKey="Lim W" first="Wilina" last="Lim">Wilina Lim</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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